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KMID : 0380219930260020132
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 2 p.132 ~ p.138
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Stimulation of Purinergic Receptor Triggers Catecholamine Secretion by a External Ca2+ Dependent Pathway in Bovine Adrenal Medulla Cells.
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Kyong-Tai Kim
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Abstract
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a rapid, transient rise in the free cytosolic Ca2+ concentration ([Ca2+)] accompanied with catecholamine secretion occurs upon treatmetn of bovine adrenal medulla (chromaffin) cells with 5 to 100 M ATP. It has been studied that the
changes
in
[Ca2+], after stimulation with ATP or other nucleotides using the Ca2+ indicator fura 2. The increased in cytosolic Ca2+ caused by ATP is mostly from an nternal store sine it is evoked in the absence of external Ca2+. This Ca2+ rise in the
cytoplasm is not dependent on the L-type voltage-sensitive Ca2+ channel since nifedipine does not block the ATP-triggered signls. UPT also elevates cytosolic Ca2+ as effectively as ATP does but catecholamine secretion was not detected.
Stmulation of cell with ATP or UTP increase the level of inositol phosphates which are known to trigger Ca2+ release form intrcellur stores. In the absence of external Ca2+, ATP-induced secretion is not occurred. ATP-triggered secretion
is
blocked by 5mM Ni2+, however nifedipine does not block secretion induced by ATP.
ATP-dependent secretion is apparently medicated by external Ca2+ entry through nifedipine-insensitive Ca2+ channel.
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